A "reagentless" fluorometric method for creatine kinase activity in serum.
نویسندگان
چکیده
Measurement of serum creatine kinase (CK; EC 2.7.3.2) activity is of great value in the diagnosis of myocardial and skeletal muscle diseases (1-3). The greater specificity of CK over that of other enzymes, such as lactate dehydrogenase and aspartate aminotransferase, for the detection of muscular dystrophy and myocardial infarction has attracted much interest in CK determinations. In a prelimina.ry study by the Sigma Co. (4) it was noted that the average increase in CK activity after an infarction is almost 1000%. We report here a “reagentless” micro-fluorometric method for the determination of serum CK. The basic reaction was first described by Oliver (5) and was subsequently improved by Rosalki (6) and Hess et al. (7). In this procedure, the “reverse” reaction of CK is coupled with two indicator reactions as follows:
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ورودعنوان ژورنال:
- Clinical chemistry
دوره 19 9 شماره
صفحات -
تاریخ انتشار 1973